Optimized Protocol for PFGE Analysis of Anginosus (milleri) Streptococci
نویسندگان
چکیده
منابع مشابه
Optimized Protocol for PFGE Analysis of Anginosus (milleri) Streptococci.
Streptococcus anginosus (milleri) is a diverse group of gram positive bacteria. Molecular methods to establish relationship between strains are poorly developed. Therefore, main tool to study genetic variability is restriction fragment length polymorphism combined with pulsed field gel electrophoresis (RFLP-PFGE). In this communication, we present optimized protocol for S. anginosus PFGE analysis.
متن کاملStreptococcus anginosus ("Streptococcus milleri"): the unrecognized pathogen.
"Streptococcus milleri" is an unofficial name that has been applied to a group of streptococci which, although basically similar, show various hemolytic, serological, and physiological characteristics. The species name Streptococcus anginosus has recently been recognized as the approved name for these organisms. Streptococci known as "S. milleri" have been implicated as etiologic agents in a va...
متن کاملStreptococcus anginosus group - cause of endocarditis and abscesses (S. milleri)
Classification, nomenclature and identification of the Streptococcus anginosus group (SAG) have been historically problematic and unreliable, mainly due to variability in bacteriological characteristics and clinical presentations. This group was first discovered in 1956 by Guthof, in reference to non-hemolytic streptococcal lassification, nomenclature and identification of the Streptococcus ang...
متن کاملPrevalence of macrolide resistance genes in clinical isolates of the Streptococcus anginosus ("S. milleri") group.
Twenty-two unrelated erythromycin-resistant anginosus group strains (3.2% resistance rate) were assessed for mechanisms of resistance. Streptococcus anginosus accounted for 16 of the 22 isolates. Fifteen isolates harbored the erm(B) gene. The erm(TR) and the mef(E) genes were carried by two isolates each. In three isolates, none of these resistance genes was detected by PCR.
متن کاملPhenotypic differentiation of Streptococcus intermedius, Streptococcus constellatus, and Streptococcus anginosus strains within the "Streptococcus milleri group".
A biochemical scheme was developed by which strains of Streptococcus constellatus, Streptococcus intermedius, and Streptococcus anginosus can reliably be distinguished from within the "Streptococcus milleri group." Strains identified as S. intermedius were differentiated by the ability to produce detectable levels of alpha-glucosidase, beta-galactosidase, beta-D-fucosidase, beta-N-acetylgalacto...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Polish Journal of Microbiology
سال: 2015
ISSN: 2544-4646,1733-1331
DOI: 10.33073/pjm-2015-008